Reconstituting Peptides

Calculate the amount of solvent needed to reconstitute your peptide.



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Reconstituting Peptides

How to Use the Calculator

Follow these steps to calculate the volume of solvent needed:

  1. Enter the peptide mass in milligrams (mg) in the “Peptide Mass” field.
  2. Enter the desired concentration in milligrams per milliliter (mg/mL) in the “Desired Concentration” field.
  3. If necessary, click on “Show Advanced Options” to provide additional information such as peptide purity, molecular weight, and unit preferences.
  4. Click on the “Calculate Volume” button to get the required volume of solvent.

Examples

Peptide Mass (mg)Desired Concentration (mg/mL)Volume of Solvent RequiredResulting Molarity (if MW = 1000 g/mol)
515 mL5.00×10-3 M
20.54 mL5.00×10-3 M
1025 mL1.00×10-2 M

Additional Information

This calculator helps you determine the volume of solvent needed to reconstitute a peptide to a desired concentration. This is essential when preparing peptide solutions for laboratory experiments or medical applications.

Notes:

  • If you know the peptide’s purity, you can enter it in the advanced options to get a more accurate calculation, adjusting the mass accordingly.
  • Providing the molecular weight allows the calculator to compute the molarity of the solution, which is important for stoichiometric calculations.
  • You can change the units of mass and volume in the advanced options to match your requirements (e.g., using micrograms (μg) and microliters (μL)).

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